Article abstract

Journal of Agricultural and Crop Research

Research Article | Published February 2021 | Volume 9, Issue 2. pp. 34-39.

doi: https://doi.org/10.33495/jacr_v9i2.21.110

 

Chemical profile and antibacterial activity of Mentha viridis L. essential oils and ethanolic extract

 



 

 

Eman Tawfik Hussien1*

Sara Abdallah Shaban2

Omar Muhammad Ali2

Helana kamil Melek Seel2

Nourhan El-Sayed Abd El-salam El-sayed2

Youssef Essam Eldeen Samir2

Galal Mohamed Remaly2

Ahmed Shaaban Mohammed2

 

Email Author
Tel: 002-01119383526

 

1. Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt.

2. Biotechnology Department, Faculty of Science, Helwan University, Cairo, Egypt.


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Citation: Hussien ET, Shaban SA, Ali OM, Seel HKM, El-sayed NEAE, Samir YEA, Remaly GM, Mohammed AS (2021). Chemical profile and antibacterial activity of Mentha viridis L. essential oils and ethanolic extract. J. Agric. Crop Res. 9(2):34-39. doi: 10.33495/jacr_v9i2.21.110.

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 Abstract 


Mentha viridis was the model plant for this study to estimate the antibacterial activity and define the essential metabolites' essential chemical compositions. This study was designed to estimate ethanol extract's chemical composition and essential oil isolated from leaves of commercial M. viridis. The essential primary and secondary metabolites were estimated and defined as: total protein (45.29 µg/ml), total alkaloids (0.116 mg/g), total flavonoids (0.9931 mg/g), and total phenolic compounds (0.2326 mg/g). The antibacterial activity of M. viridis ethanol extract was screened against two-gram negative bacterial strains, Pseudomonas aeruginosa and Escherichia coli (DH5-α). This work proved that both the ethanol extract and the essential oil extract of M. viridis leave have potent antibacterial activity against tested bacteria. There is a difference in clear zone diameter by comparing the control ethanol treatment to the ethanol extract. The total essential oil obtained by hydro-distillation technique and identified by Gas chromatography-mass spectrometry. The main compounds of the oil were Cyclohexanone, 5-methyl-2-(1-methylethylidene)- (33.46%), D-Carvone (32.30%), Cyclohexanol, 2-methyl-5-(1-methylethenyl)-, (1.alpha., 2.beta., 5.alpha.) (7.13%), Carveol (5.31%), Dihydrocarvyl acetate (5.30%). All these characterizations enable M. viridis to have antibacterial, antioxidant, anti-inflammatory and anticarcinogenic effects.

Keywords  Antibacterial activity   essential oils   ethanol extract   gas chromatography-mass spectrometry (GC-MS)    Mentha Viridis 

 

 

Copyright © 2021 Author(s) retain the copyright of this article.or(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

 

 

  References 

 

Adams RP (2001). Identification of essential oil components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corp. Illinois.

 

Balla OY, Ali MM, Garbi MI, Kabbashi AS (2017). Chemical Composition and Antimicrobial Activity of Essential Oil of Mentha viridis. Biochem. Mol. Biol. 2(5):60-66. doi: 10.11648/j.bmb.20170205.12.

 

Bradford MM (1976). A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem. 72:248-254.

 

Braun L, Cohen M (2014). “Herbs and Natural Supplements”. Volume 2: An Evidence-Based Guide: Elsevier Health Sciences.

 

Brown ED, Wright GD (2016). “Antibacterial drug discovery in the resistance era”. Nature 529(7586):336-343.

 

Fonkeng LS, Mouokeu RS, Tume C, Njateng GS, Kamcthueng MO, Ndonkou NJ, Kuiate JR (2015). Anti-Staphylococcus aureus activity of methanol extracts of 12 plants used in Cameroonian folk medicine. BMC Research Notes, 8(1):1-6.

 

Harborne JB (1973). Methods of plant analysis. In: Phytochemical Methods. Chapman and Hall, London.

 

Gaurav N (2016). “An Experimental Text Book on Phytochemical Analysis and Antimicrobial Activity of Mentha Piperita”. Onlinegatha.

 

Hassan HA, Kabbashi AS, Abedallah A, Wagh VD, Hamdi OAA (2019). Chemical Composition, Antioxidant Activity and Cytotoxicity of Essential Oil of Mentha viridis. ACTA Scientific Medical Sciences (ASMS). 3(8):200-205.

 

Kalemba D, Kunicka A (2003). Antibacterial and antifungal properties of essentials oils. Curr. Med. Chem. 10(10):813-839.

 

Kujala TS, Loponen JM, Klika KD, Pihlaja K (2000). Phenolics and betacyanins in red beetroot (Beta vulgaris) root: distribution and effect of cold storage on the content of total phenolics and three individual compounds. J. Agric. Food Chem. 48(11):5338-5342.

 

Lawrence BM (2006). Mint: The Genus Mentha. CRC Press, Boca Raton, FL.

 

Lixandru B, Dracea N, Dragomirescu E, Coldea I, Dobre E, Rovinaru C (2010). Antimicrobial activity of plant essential oil against bacterial and fungal species involving in food poisoning. Cantacuzino “National Institute of Research-Development of Microbiology and Immunology, Bucharest, Romania.

 

Nakatani N (1994). Antioxidative and Antimicrobial Constituents of herbs and spices. Spices, Herbs and Edible Fungi. Elsevier Science B. V. 251-271.

 

Okigbo RN, Mmeka EC (2008). Antimicrobial effects of three tropical plant extracts on Staphylococcus aureus, Escherichia coli and Candida albicans. Afr. J. Tradit. Complement Altern. Med. 5:226-229.

 

Ouakouak H, Chohra M, Denane M (2015). “Chemical composition, antioxidant activities of the essential oil of Mentha pulegium L., South East of Algeria”. Int. Lett. Nat. Sci. 39:49-55.

 

Peter K V (2001). Hand book of Herb and Spices. Wood head publishing limited, Abington. Rita P, Animesh DK (2011). An update overview on peppermint (Mentha piperita L.). Int. J. Pharm. Pharm. Sci. 8(2):1-10.

 

Shaikh S, Yaacob HB, Rahim ZHA (2014). Prospective role in treatment of major illnesses and potential benefits as a safe insecticide and natural food presertive of mint (Mentha spp.): a review. Asian J. Biomed. Pharm. Sci. 4(35):1-12.

 

Silva LF, Braga MA, Espósito MA, Cesar PHS, Marcussi S (2019). Inhibition by essential oils of Mentha viridis and Mentha pulegium (Lamiaceae) in proteolysis, fibrinogenolysis and coagulation caused by venomous snakes. Rev. Biol. Trop. 7(4): 999-1009.

 

Yonis YM, Beshir SM (2004). Carvone rich essential oil from Mentha longifolia (L) Huds. Ssp. Schimperi Briq. and Mentha spicata L. grown in Sudan. J. Essent. Oil Res. 16:39-541.

 

Zhishen J, Mengcheng T, Jianming W (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64:555-559.

 

Zuzarte M, Goncalves MJ, Cavaleiro C, Canhoto J, Vale-Silva L, Silva MJ, Pinto E, Salgueiro L (2011). Chemical composition and antifungal activity of the essential oils of Lavandula viridis L’He´r. J. Med. Microbiol. 60:612-618.