Article abstract

Journal of Agricultural and Crop Research

Research Article | Published September 2021 | Volume 9, Issue 9. pp. 212-221.

doi: https://doi.org/10.33495/jacr_v9i9.21.148

 

Mycorrhizae as a biological method for improving soil fertility and controlling Rhizobium-inoculated soybean collar rot disease in Benin

 



 

 

Howell B Houngnandan1

Adélaïde H. Fagnibo2

Appolinaire Adandonon1, 2*

Leslie DR Bossou1

Mahougnon Charlotte C Zoundji2

Félix Kouèlo3

Tobi Moriaque Akplo3

Pascal Houngnandan1, 2, 3

 

Email Author


 

1. Laboratory of Plant, Horticultural and Forest Sciences (LaSVHF), National University of Agriculture (UNA), Republic of Benin.

2. School of Crop and Seed Production and Management, National University of Agriculture of Porto Novo; BP 43 Kétou, Republic of Benin.

3. Laboratory of Soil Microbiology and Microbial Ecology, Faculty of Agronomic Sciences, University of Abomey-Calavi (UAC); 01 BP 711 Abomey-Calavi, R. Benin.


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Citation: Houngnandan HB, Fagnibo AH, Adandonon A, Bossou LDR, Zoundji MCC, Kouèlo F, Akplo TM, Houngnandan P (2021). Mycorrhizae as a biological method for improving soil fertility and controlling Rhizobium-inoculated soybean collar rot disease in Benin. J. Agric. Crop Res. 9(9):212-221. doi: 10.33495/jacr_v9i9.21.148.

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 Abstract 


The current study aims to improve soybean productivity without mineral fertilizer inputs by testing the effect of indigenous species of arbuscular mycorrhizal fungi (AMF) in double inoculation with Bradyrhizobium on soybean collar rot (SCR). The trial was conducted in North Benin and included 11 treatments, namely eight AMF species in double inoculation with Bradyrhizobium and three controls, namely Bradyrhizobium + 100 kg.ha-1 of P2O5 (RP100), Bradyrhizobium without mycorrhiza (RM0) and the untreated control without either Bradyrhizobium or mycorrhiza (R0M0). The results showed R0M0 to have the highest incidence of collar rot disease. The causal agent of the disease was identified in the current study as Sclerotium rolfsii. When not treated, the disease incidence in the current study was more than 70%, while, when treated with AMF as Racocetra crispa, Gigaspora margarita, Scutellospora savannicola, Paraglomus occultum, Rhizophagus partial (Unc) and Diversispora sp., this disease incidence was significantly (p < 0.05) as low as 6% and was not more than 27%. The treatment RP100 as positive control also resulted in a disease incidence lower than 27%. The AMF species Racocetra crispa gave the highest plant height while R. crispa, P. occultum, S. savannicola and R. partial (Unc) gave the highest grain yields, best symbiotic parameters and high soil phosphorus and nitrogen levels at harvest. This is the first report of S. rolfsii-caused collar rot disease in soybean in Benin and also of the effect of indigenous arbuscular mycorrhizal fungi on the disease incidence.

Keywords  Bradyrhizobium   collar rot disease   Mycorrhizations   Sclerotium rolfsii   soybean 

 

 

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This article is published under the terms of the Creative Commons Attribution License 4.0

 

 

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